Small P particles formed by the Taiwan-native norovirus P domain overexpressed in Komagataella pastoris
نویسندگان
چکیده
منابع مشابه
Compact multi-enzyme pathways in P. pastoris.
We report for the first time the functional simultaneous expression of nine genes from a single 2A peptide based polycistronic expression construct. The feasibility and arising opportunities for the biosynthetic pathway balancing for chemical production were demonstrated by the co-expression of the violacein and carotenoid biosynthesis pathways.
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Background: Bovine follicle-stimulating hormone (bFSH) is a heterodimer hormone that consists of a common -subunit which noncovalently associated with the hormone-specific -subunit. During the past 15 years, the methylotrophic yeast Pichia pastoris has become an important host organism for recombinant protein production because it is able to use methanol as a sole carbon and energy source. Th...
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Answering a question of D.Dikranjan and I.Protasov we prove that each infinite group G contains a weakly P-small subset A ⊂ G which is not P-small. This means that for every n there are n pairwise disjoint translation copies of A in G but there is no infinitely many such disjoint copies. In this paper we answer a question of D.Dikranjan and I.Protasov concerning the relation between P-small and...
متن کاملSecreted production of assembled Norovirus virus-like particles from Pichia pastoris
BACKGROUND Norovirus virus-like particles (NoV VLPs) have recently been explored as potential vaccine platforms due to their ability to produce an effective immune response. Expression of the main structural protein, VP1, leads to formation of self-assembled particles with similar characteristics to the original virus. These NoV VLPs have been expressed in Escherichia coli, yeast and insect cel...
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The cytoplasm-to-vacuole targeting (Cvt) pathway of Saccharomyces cerevisiae delivers aminopeptidase I (Ape1) from the cytosol to the vacuole, bypassing the normal secretory route. The Cvt pathway, although well-studied, was known only in S. cerevisiae. We demonstrate its existence in the methylotrophic yeast, Pichia pastoris, where it also delivers P. pastoris Ape1 (PpApe1) to the vacuole. Mos...
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ژورنال
عنوان ژورنال: Applied Microbiology and Biotechnology
سال: 2018
ISSN: 0175-7598,1432-0614
DOI: 10.1007/s00253-018-9331-8